近日，真迈生物自主研发的创新技术再次获得国际认可，包括全内反射荧光成像系统在内的三项发明专利正式通过欧洲专利局（European Patent Office）的授权批准，成为有效欧洲专利。
至此，历经多年自主研发积累，真迈生物目前拥有的国内外专利（含授权有效和申请中）及计算机软件著作权登记等已近200件。
01
METHOD FOR CONTROLLING BASE SEQUENCE DETERMINATION REACTION, BASE SEQUENCE DETERMINATION SYSTEM AND CONTROL DEVICE
（对序列测定反应进行控制的方法、序列测定系统和控制装置）
技术简介：
序列测定系统包括流体装置和光学装置，反应装置包括第一单元和第二单元，定义序列测定反应包含的一种重复执行单位为第二生化反应-第一生化反应-拍照，方法包括在完成初始步骤之后，使得当利用流体装置使第一单元和第二单元中的一个上的样品进行第二生化反应和第一生化反应的同时，利用光学装置对另一个单元上的样品进行拍照。初始步骤包括：a）利用流体装置使第一单元和第二单元中的一个进行样品的第一生化反应，b）利用光学装置对进行第一生化反应后的单元的样品的进行拍照，c）利用流体装置使第一单元和第二单元的另一个进行样品的第一生化反应。上述方法可提高序列测定效率。
“The present disclosure discloses a method for controlling a base sequence determination, a base sequence determination system, and a control device. The base sequence determination system includes a fluid device and an optical device, a reaction device includes a first component and a second component, and a repeated executable unit included in the base sequence determination is defined as: a second biochemical reaction—a first biochemical reaction—photographing. The method includes, after initiation steps are completed, using the fluid device to perform the second biochemical reaction and the first biochemical reaction of the sample on the first component, while using the optical device to photograph the sample on the second component. The initial steps include: a. using the fluid device to perform the first biochemical reaction of the sample on the first component, b. using the optical device to photograph the sample on the first component after the first biochemical reaction, and c. using the fluid device to perform the first biochemical reaction of the sample on the second component. The above-mentioned method can improve the efficiency of base sequence determination.”
02
SINGLE-MOLECULE IMAGE CORRECTION METHOD, DEVICE AND SYSTEM, AND COMPUTER-READABLE STORAGE MEDIUM
（单分子图像纠偏方法及装置）
技术简介：
本专利公开了一种单分子图像纠偏方法及装置，其中方法包括：根据第一图像的每一像素点对应的亮度值矩阵，获取与所述第一图像对应的第一目标简化矩阵，并根据第二图像的每一像素点对应的亮度值矩阵，获取与所述第二图像对应的第二目标简化矩阵；对所述第一目标简化矩阵和所述第二目标简化矩阵分别进行二维离散傅里叶变换，得到与所述第一目标简化矩阵对应的第一傅里叶矩阵，并得到与所述第二目标简化矩阵对应的第二傅里叶矩阵；根据所述第一傅里叶矩阵和所述第二傅里叶矩阵获取所述第二图像相对于所述第一图像的偏移量；根据所述偏移量对所述第二图像进行纠偏。可见，实施本发明能够对不同时刻拍摄的单分子图片进行纠偏。
“An embodiment of the present disclosure discloses a method and a device of correcting a single-molecule image, the method includes: acquiring the first target simplified matrix corresponding to the first image according to the intensity matrix corresponding to each of pixels in the first image, and acquiring the second target simplified matrix corresponding to the second image according to the intensity matrix corresponding to each of the pixels in the second image; the two-dimensional DFT is performed to the first target simplified matrix and the second target simplified matrix respectively to acquire the first Fourier matrix corresponding to the first target simplified matrix and the second Fourier matrix corresponding to the second target simplified matrix; the method of correcting a single-molecule image acquire the offset of the second image with respect to the first image according to the first Fourier matrix and the second Fourier matrix; and correcting the second image according to the offset. Such method can correct the images of single molecules taken at different moments.”
03
TOTAL INTERNAL REFLECTION FLUORESCENCE IMAGING SYSTEMAND SEQUENCING DEVICE
（全内反射荧光成像系统及测序装置）
技术简介：
本专利公开了一种全内反射荧光成像系统及测序装置，全内反射荧光成像系统包括第一成像系统和第二成像系统，所述第一成像系统包括N条独立的激光光路，所述第二成像系统通过第二接收装置获取到第二像的位置的不同判断样品高度的变化，根据样品高度的变化进行自动补偿，以保证样品始终处于聚焦的位置。本发明可通过第二成像系统分别对N条独立的激光光路进行光路调节，以实现N条独立的激光光路具有相同的穿透深度，进而无需在切换不同光源时，再进行光路调节，从而节省了实验当中费时的光路调节。
“The present disclosure discloses a total internal reflection fluorescence imaging system and a sequencing device. The total internal reflection fluorescence imaging system includes a first imaging system and a second imaging system. The first imaging system includes N separate laser light paths. The second imaging system determines the changes of the height of the sample by means of the differences of the positions at which the second receiving device acquires the second images, and performs automatic compensation according to the changes of the height of the sample, so as to ensure that the sample is always at a focused position. The embodiment can perform light path adjustment of the N separate laser light paths respectively by means of the second imaging system, so as to realize that the N separate laser light paths have the same penetration depth. Therefore no further light path adjustment is required when switching between different light sources, thus saving the time consuming for light path adjustment.”
自主研发、科技创新是真迈创立之初的内在基因，也是真迈持续发展至今的战略根基。为了有效支撑公司科技创新战略，包括有效地对无形资产进行转化和管理运营，公司一直秉承尊重科学家和工程师的团队文化，并据自身情况设立专门部门和人员进行知识产权管理，公司知识产权的管理运行符合国家推荐性标准GB/T 29490-2013《企业知识产权管理规范》。未来，真迈将进一步加强深化知识产权布局，持续促进公司核心竞争力的稳步提升。